No 07+08/2006 » Abstract

Surveyor™ Nuclease-Based Genotyping of SNPs

by Noriaki Mitani, Shinobu Tanaka, Yasuyuki Okamoto

Surveyor™ nuclease, a single-strand-specific endonuclease that cleaves DNA at the 3' side of a base-mismatch site in both DNA strands, can be used for genotyping of SNPs by preparing two different types of a heteroduplex: one from mutant DNA fragments and the other from a mixture of wild-type and mutant fragments. We show an example of this technique and propose that it can be used with some advantages instead of RFLP analysis.

Clin. Lab. 2006;52(7-8):385-386.

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