No 07+08/2010 » Abstract

Comparison of Four Direct Homogeneous Methods for the Measurement of Low-Density Lipoprotein Cholesterol

by Koji Yamada, Naoki Tsuji, Tomoko Fujita, Maki Tanaka, Kageaki Kuribasyashi, Daisuke Kobayashi, Naoki Watanabe

Background: The primary lipoprotein risk factor is low-density lipoprotein cholesterol (LDL-C), and medication is targeted at lowering LDL-C values. To clarify the usefulness of direct homogeneous assays for LDL-C measurement, we compared the values obtained by various reagents to those obtained by the Friedewald equation and analyzed different reactivity to IDL/VLDL and LDL.
Methods: Serum samples were collected from 55 patients with hypercholesterolemia. The LDL-C concentrations were determined by four direct homogeneous assays using reagent A (Kyowa Medex), B (Sekisui Medical), C (Denka Seiken), and D (Sysmex), which are commercially available.
Results: Significant correlation was observed in LDL-C values obtained by the homogeneous assays and the Friedewald equation. However, there were two discrepancies in reagents B and C, respectively. These assays showed 40 % and 55 % lower LDL-C values than those calculated by the Friedewald equation, respectively. Reactivity to the IDL fraction in reagents B and C was lower than in reagents A and D.
Conclusions: Direct homogeneous assays for LDL-C are suitable for routine laboratory examination. However, it was shown that attention should be given to the different reactivity to IDL and LDL among reagents in some clinical samples.

Clin. Lab. 2010;56(7-8):327-333.

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