No 07+08/2011 » Abstract

Presence of Murine Leukemia Virus (MLV)-related Virus Gene Sequences in a Commercial RT-PCR Reagent

by Dietmar Wolff, Andreas Gerritzen

Background: The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients.
Methods: We wanted to establish a MLV-related virus real-time PCR for routine diagnostics.
Results: Our study identified false positive MLV-related virus results due to a contamination of Superscript III Platinum One-Step Quantitative RT-PCR System (Invitrogen).
Conclusions: This observation may be helpful to elucidate discrepant results for the detection of MLV-related virus like xenotropic MLV-related virus (XMRV) in recently published studies.

Clin. Lab. 2011;57(7-8):631-634.

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