Clinical Laboratory

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Abstract	Highly Sensitive and Efficient Screening of Streptococcus Agalactiae Through Improved Real-Time PCR Protocols and Optimized Analytical Parameters by DS Winkler, A Rocha, CD Fabiao, M De Marco, R Proto-Siqueira

	Background: The detection of Streptococcus agalactiae colonization during pregnancy is important in order to prevent neonatal infections and reduce mortality rates. Methods: A highly sensitive (up to 1,000 UFC/mL), fully automated, and cost effective cylB specific real-time PCR protocol was developed for the detection of S. agalactiae in anal and vaginal swabs of pregnant women. Results: From the 62 test subjects, 24 were considered positive by PCR (38%) and 14 by culture (24%; p = 0.0015). Conclusions: The observed discrepancies and the reduced turnaround time justifies molecular screening for S. agalactiae. DOI: 10.7754/Clin.Lab.2015.150221

Abstract