You have to be registered and logged in for purchasing articles.

Abstract

SHORT COMMUNICATIONEffects of Storage Conditions on Urinary LH and FSH Measurement Using Immunochemiluminometric Assay by Yaping Ma, Zhuangjian Xu, Guixiong Gu, Feng Ren, Hua Jin, Wenying Zhu

Background: Urine is a good alternative body fluid for gonadotropin studies. There was limited information about the effects of different storage conditions on urinary gonadotropin measurement by using immunochemiluminometric assay (ICMA).
Methods: ICMA was used to determine gonadotropin in urine stored under different conditions, such as different pH, storage time, and cycles of freeze-thaw.
Results: Luteinizing hormone (LH) level was not significantly affected at pH 2.5 to 10.5 or being stored at 4°C for 3 days. Follicular stimulating hormone (FSH) level was not significantly changed at pH 3.5 to 10.5 or throughout 49-day storage at 4°C in the absence of glycerol. LH was significantly decreased after freeze-thawing twice, while FSH was resistant to freeze-thaw procedures.
Conclusions: LH and FSH can be determined by ICMA in normal urine pH range (4.6 ~ 8.0). Urine LH is more sensitive to long-term storage and multiple freeze-thaw procedures than FSH.

DOI: 10.7754/Clin.Lab.2017.171011