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Second Allele Finder Software: a Simple Approach Toward HLA Typing by Zeinab Fadaie, Marzieh Ahmadzadeh, Mohammad Salehi, Mohammadreza Bordbar, Majid Fardaei

Background: Human Leukocyte Antigen (HLA) has an important role in presenting self and non-self-antigens to T-cell receptors on T lymphocytes. For tissue transplantation and the possibility of graft rejection, these HLA antigens on the surface of the donor and recipient's cells should be checked.
Methods: In this study, a novel technique was used for HLA typing by cloning and sequencing. The most polymorphic exons of HLA-A, HLA-B, and HLA-DRB1 in five unrelated persons were cloned into the T-vector. Afterward, the sequencing data were analyzed using a new computer software named SAF (Second Allele Finder), which was designed for this study.
Results: Using this new technique, the HLA typing was performed in five unrelated persons, for four of them the HLA typing was done by the PCR-SSP method. Despite that the PCR-SSP method is typing in the first field, the results of this study were obtained in the third field, which is more valid and accurate.
Conclusions: This study presents a new, accurate, and low cost technique for typing HLA loci in tissue transplantation especially in bone marrow transplantation.

DOI: 10.7754/Clin.Lab.2016.160220