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The pre-mir-499 Variant rs3746444 May Contribute to Coronary Artery Disease Susceptibility: a Case-Control and Meta-Analysis Study by Afsaneh Labbaf, Hamid Ghaedi, Behnam Alipoor, Mir Davood Omrani, Faranak Kazerouni, Mehrnoosh Shanaki, Maryam Ghaffarzadeh, Hossein Pashaiefar, Ali Rahimipour

Background: Recent reports have suggested that single-nucleotide polymorphisms (SNPs) in microRNA genes may contribute to individual susceptibility to coronary artery disease (CAD). The purpose of this study was to evaluate the association between rs3746444 in pre-miR-499 with CAD.
Methods: We performed a case-control study including 288 CAD patients and 150 control subjects. DNA was extracted from blood samples and genotyped through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Meta-analysis was performed under fixed effect and random effect models whenever appropriate.
Results: We found that the GG genotype is significantly more frequent in CAD patients than controls (adjusted p = 0.010; OR = 1.99, 95%; CI: 1.18 - 3.38). Additionally, through a meta-analysis, we showed that miR-499rs3746444 has a significant association with cardiovascular disease.
Conclusions: Our results suggest that miR-499-rs3746444-GG is associated with CAD susceptibility and development.

DOI: 10.7754/Clin.Lab.2016.161011