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Residual Negative Pressure in Vacuum Tubes Might Increase the Risk of Spurious Hemolysis by Tong-Tong Xiao, Qiao-Xin Zhang, Jing Hu, Hui-Zhen Ouyang, Ying-Mu Cai

Background: We planned a study to establish whether spurious hemolysis may occur when negative pressure remains in vacuum tubes.
Methods: Four tubes with different vacuum levels (-54, -65, -74, and -86 kPa) were used to examine blood drawn from one healthy volunteer; the tubes were allowed to stand for different times (1, 2, 3, and 4 hours). The plasma was separated and immediately tested for free hemoglobin (FHb). Thirty patients were enrolled in a verification experiment.
Results: The degree of hemolysis observed was greater when the remaining negative pressure was higher. Significant differences were recorded in the verification experiment.
Conclusions: The results suggest that residual negative pressure might increase the risk of spurious hemolysis.

DOI: 10.7754/Clin.Lab.2017.161125