Bacground: Recently it has been claimed that single nucleotide polymorphism (SNP) near the interleukin 20 (rs1518108) gene is associated with susceptibility to HCV infection. Therefore, in this study, we determined the distribution of the rs1518108 (C/T) polymorphism with susceptibility to hepatitis C and also sustained virological response (SVR) to chronic hepatitis C virus infection among Iranian patients under PEG-IFN combined with ribavirin theraphy.
Methods: This case-control study, was performed on 136 blood samples based on 81 patients with chronic HCV genotypes 1 and 3 including 64 SVR positive and 17 negative and 55 healthy individual controls. DNA was isolated from the samples and the frequency of the polymorphism was analyzed using a PCR-RFLP method. Finally, the products were detected on 3.5% agarose gel electrophoresis.
Results: The analysis of the data for C/T polymorphism indicated that the CC genotype was found in 19 of 64 patients who achieved SVR, while the TT genotype was detected in 3 patients and SVR was achieved in 2. Finally, heterozygous CT was identified in 53 patients and 10 patients were resistant to treatment.
Conclusions: The results did not support any significant effects of TT or CT genotypes on susceptibility to HCV infection (p = 0.935, OR = 1.031, CI = 0.464 - 2.026). Moreover, there was no significant correlation between SVR to PEG-IFN combined by ribavirin therapy in patients with genotype CC (p = 0.601, OR = 0.736, CI = 0.234 - 2.319).