Abstract

Background: Wahl's macroglobulinemia (WM) is a disease in which plasma cells proliferate abnormally and secrete high concentrations of monoclonal immunoglobulins, mainly IgM-type immunoglobulins, leading to a series of symptoms in patients. IgM can interfere with our laboratory results. In this paper we innovatively use a zero-input approach to eliminate this interference and evaluate it.
Methods: We cleverly utilized the principle that IgM precipitates in contact with water. The solution is then centrifuged to remove the precipitate and the supernatant is then tested.
Results: Ultimately, the results obtained using our method were in good agreement after comparison with stronger anti-interference performance but costly reagents.
Conclusions: Our proposed zero-input method is able to remove the IgM interference very well that is commonly found in laboratories. The method is simple and easy to operate with almost zero input, which makes it very suitable for generalization.

DOI: 10.7754/Clin.Lab.2025.250330