Abstract
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Comparison of the Autof MS1000 with the Vitek MS in the Identification of Bacterial and Yeast Isolates
by Neslihan Arici, Melih T. Özdemir, Nurver Ülger, Damla Köklü, Nilgün Kansak, Riza Adaleti, Handan Ankarali, Sebahat Aksaray
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Background: Matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF) has been widely used in clinical microbiology laboratories as a rapid and reliable tool for pathogen identification. The aim of this study was to evaluate the diagnostic performance of the newly-developed Autof MS1000 in comparison with the Vitek MS.
Methods: A total of 578 clinical isolates consisting of 136 Enterobacterales, 76 non-fermenting Gram-negative bacilli, 17 other Gram-negative bacilli, 240 Gram-positive cocci, 30 Gram-positive bacilli, 52 anaerobic bacteria, and 27 yeasts were tested simultaneously by the two systems. The direct smear method was performed for bacteria and the formic acid extraction method for yeasts. Discrepant results were confirmed by 16S rRNA or ITS region sequencing.
Results: The Autof MS1000 and Vitek MS identified 93.3% and 95.5% of the strains at the species level, respectively. Three isolates (0.3%) yielded “no identification” results with Vitek MS, and no “unreliable” results (0%) were obtained with Autof MS1000. The Autof MS1000 and Vitek MS misidentified 1.5% and 1.4% of the isolates, respectively. Overall, there was significant agreement between the two systems (p < 0.001). In terms of identification times, the Autof MS1000 was approximately three times faster than the Vitek MS.
Conclusions: Our results demonstrate that the Autof MS1000 provides comparable results to the Vitek MS and can be used for the rapid identification of microorganisms. Furthermore, this study highlights the need for any MALDITOF MS system to implement regular database expansion for the identification of rarely encountered microorganisms.
DOI: 10.7754/Clin.Lab.2025.250430
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