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Maternal Plasma Advanced Glycation End Products Concentrations in Response to Oral 50-gram Glucose Load in Mid-Pregnancy: A Pilot Study by Michelle A. Williams, Daniel A. Enquobahrie, Jennifer Zimmer, Chun Fang Qiu, Karin Hevner, Dejene Abetew, Chad Christianson, Chrystal Sheaff

Background: Accumulating evidence documents the initiation of diverse physiologic and biochemical responses subsequent to an oral glucose load.
Objectives: We sought to evaluate the extent to which acute hyperglycemia, resulting from a 50-gram glucose load, contributes to changes in maternal plasma concentrations of advanced glycation end products (AGEs), a heterogeneous group of molecules formed from the non-enzymatic reaction of reducing sugars with free amino groups of proteins, lipids, and nucleic acids.
Methods: Blood specimens were collected from each participant in mid-pregnancy using standard procedures before and after a 50-gram oral glucose load. Maternal plasma methylglyoxal (MG), pentosidine and Nε-(carboxymethyl)lysine (CML) (free and bound) were measured by HPLC-MS/MS method. Non-parametric methods were employed for statistical analysis.
Results and Conclusions: Median plasma MG increased 1.27 fold as a result of acute hyperglycemia. Median bound CML concentrations were elevated 21% in post-load plasma samples as compared with preload samples, while median free pentosidine concentrations were 51% lower (both p-values < 0.05). Future studies of larger populations and longer periods of follow-up are warranted to investigate the consequences of acute and chronic hy-perglycemia on placental function and fetal development.

DOI: 10.7754/Clin.Lab.2012.111219